一、细胞基本属性
|
|
细胞名称
|
OCI-LY3(人弥漫大B细胞淋巴瘤细胞)
|
细胞别称
|
OCI-LY3; OCI-ly3; OCI-LY-3; Oci-Ly-3; OCI-Ly 3; OCILY-3; OCI-Ly03; OCI Ly3; OCILY3; Ly3; LY3
|
商品货号
|
ORC0985
|
种属来源
|
人
|
年龄性别
|
男,52岁
|
组织来源
|
肿瘤细胞
|
生长特性
|
悬浮细胞
|
细胞形态
|
圆形细胞,聚团生长
|
特征特性
|
established in 1983 from the bone marrow sample of a 52-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large B-cell lymphoma, DLBCL, stage 4B) at relapse; cells were described in the literature to be EBV-negative and lacking a classical t(8;14) translocation. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
|
生物安全等级
|
1
|
细胞规格
|
1×106cells/T25培养瓶或者1mL冻存管包装
|
支原体检测
|
无
|
培养基
|
RPMI-1640+20% FBS+1% P/S
|
培养条件
|
气相:95%空气+5%二氧化碳;温度:37℃
|
冻存条件
|
冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
|
倍增时间
|
~24 hours (DSMZ)
|
传代比例
|
1:2-1:4
|
换液频率
|
2~3天/次
|